Journal of Environmental Treatment Techniques  
2020, Volume 8, Issue 3, Pages: 1081-1083  
J. Environ. Treat. Tech.  
ISSN: 2309-1185  
Journal web link: http://www.jett.dormaj.com  
Formulation of Microorganisms for Cane Plants  
Ririen Prihandarini* and Sudiarso  
Lecturer at the Faculty of Agriculture, University of Widyagama Malang, Indonesia  
Received: 25/03/2020  
Accepted: 29/06/2020  
Published: 20/09/2020  
Abstract  
Sugar is one of the strategic commodities in the Indonesian economy. With an area of around 450 thousand hectares in the 2007-  
017 period, the sugar cane-based sugar industry is one of the sources of income for around 900 thousand farmers with the number of  
2
workers involved reaching around 1.3 million people. Sugar is also one of the basic needs of the community; the dynamics of sugar  
prices will have a direct influence on the inflation rate. One effort that can be done to improve sugarcane productivity is to improve  
soil fertility through sugarcane cultivation techniques by engineering microorganism technology. The potential of nature and the  
wealth of microorganisms in Indonesia are numerous but have not been explored and utilized to the full Engineering the use of  
microorganisms has good prospects for restoring land degradation in sugarcane agroecosystem. Microorganism-formulation was  
designed to get the most suitable microorganism combination for sugarcane. Various types of Nitrogen-fixing microorganisms such as  
Nitrobacter, Nitrosomonas, Nitrosococcus, and Rhizobium will be combined with Phosphate solvent bacteria such as Pseudomonas,  
Bacillus, Pseudomonas, Xanthomonas, Acetobacter Thiobacillus, Thrichodema and some decomposer bacteria. Found the most  
suitable formulation for the growth of sugarcane seedlings, formulation of the microorganisms Sacharomyces (S), Azospirillum sp (A),  
Azotobacter (Z), Rhizobium (R), Pseudomonas (P), Bacillus dan Trichoderma sp (T) which is formulated in a microorganism  
technology with the popular name Refresh Microorganism (RIM).  
Keywords: Cane, Formulations, Microorganisms  
Introduction1  
provide nutrients according to plant requirements have been  
1
obtained [4], only still need to be modified specifically for  
sugarcane. The soil health index of Indonesia must also be  
considered for better plant requirements achieved [5-7]. The  
purpose of this study is to formulate a consortium of  
microorganisms that can help sugarcane plants to grow better.  
Dependence on chemical fertilizers causes chemical  
fertilizer subsidies to increase every year. Environmental  
burden caused by the use of chemical fertilizers continuously  
in high doses increasingly close the pores of the soil, so that  
the absorption of rainwater is greatly decreased and resulting  
in frequent floods and drought [1]. In addition, the use of  
chemical fertilizers continuously causes the soil to become  
hard so that the main crop production of sugar cane becomes  
smaller and the yield of sugar obtained is low [2]. It is time  
for our farming system to return to nature with organic  
farming. Microorganism technology will help organic farming  
systems to be realized easily, quality and inexpensive so it  
must be prepared on an industrial scale [3].  
Synergy between microorganism technologies intended  
for the development of organic agriculture and sugar cane  
farmers as users is something that needs to be socialized and  
implemented in the field. For this reason, continuous research  
is needed. The potential of microorganisms in Indonesia is  
extraordinary in terms of species, quantity and function; this is  
due to the fact that in Indonesia, 2 of these mountain ranges in  
the world meet, in Indonesia located between 3 continents and  
the meeting of the Indian and Pacific oceans.  
2 Materials and Methods  
This research was conducted from April in the Laboratory  
of the Department of Agrotechnology, Faculty of Agriculture,  
Brawijaya University Malang. This formulation research was  
carried out by mixing various pure cultures of photosynthetic  
bacteria Sacharomyces (S), Azospirillum sp (A), Azotobacter  
(Z), Rhizobium (R), Pseudomonas (P), Bacillus (B) and  
Trichoderma sp (T) with natural ingredients such as banana  
weevil and sugar cane shoots that contain a variety of natural  
hormones. The formulation trials are used on sugarcane  
seedlings, the seeds that grow best will be found with the  
fastest growing indicator of shoots.  
3
Results and Discussion  
Based on the synergy test between a consortium of  
microorganisms  
between  
photosynthetic  
bacteria  
This has the opportunity to build a microorganism  
industry that has the opportunity to increase crop production  
and quality, especially sugar cane. Reliable pioneer  
microorganisms that have been selected by the research team  
since the 1990s in this study combined into a force that can  
help sugarcane plants to produce high yields with high yields.  
Pioneering microorganism formulations that are reliable  
in improving polluted environments and helping plants  
Sacharomyces (S), Azospirillum sp (A), Azotobacter (Z),  
Rhizobium (R), Pseudomonas (P), Bacillus (B) and  
Trichoderma sp (T) with a comparison, the formula obtained  
F1, F2, F3, F4 and F5 that are used can synergize with one  
another. From the results of incubation for 24 hours, each  
formula contained dominance and which could blend (Table  
1
).  
3
.1 Antagonist  
Bacterial consortium is a collection of bacteria that work  
together to form a community, to produce significant  
products.  
Corresponding author: Ririen Prihandarini, Lecturer at the  
Faculty of Agriculture, University of Widyagama Malang,  
Indonesia. E-mail: ririenuwg@gmail.com.  
1081  
Journal of Environmental Treatment Techniques  
2020, Volume 8, Issue 3, Pages: 1081-1083  
The compatibility or synergism of two or more inoculated  
bacteria is a very important factor so that the bacteria can  
work well together. Bacteria with the same genus or species  
can interact and synergize, and share sources of nutrients that  
can be donated to their environment.  
Table 1: Synergy between Azotobacter isolates  
Formula  
R
+
+
+
+
+
+
+
+
S
+
+
+
+
+
+
+
+
A
+
+
+
+
+
+
+
+
Z
+
+
+
+
+
+
+
+
P
+
+
+
+
+
+
+
+
B
+
+
+
+
+
+
+
+
T
+
+
+
+
+
+
+
+
R
S
A
Z
L
P
F1  
F3  
F5  
F2  
B
T
+
: Synergistic  
Among the 5 formulas above can be seen how the  
synergy, dominance and speed of growth of each  
microorganism. Specifically the speed of growth of each  
microorganism is obtained by seeing the movement of  
microorganisms and their propagation directly using  
hanging preparation on a microscope connected to the  
television layer. Between 5 formulas, it turns out that there are  
a
F4  
3
Formula which are better for the cooperation of  
microorganisms with indicators of the spread of  
microorganisms and there is no domination of  
microorganisms chosen F1, F4 and F5.  
Figure 1: Observation of a 5 Formula microorganism consortium  
S0 (Control)  
S1  
S2  
S3  
Figure 2: Sugarcane seedlings tested with various selected formulas  
1082  
Journal of Environmental Treatment Techniques  
2020, Volume 8, Issue 3, Pages: 1081-1083  
3
.2 Direct testing with sugarcane seedlings  
5. Latif Maher R. Technical development to assess soil health index in  
Indonesia. Journal of Applied and Physical Sciences, 2018; 4(3): 79-  
Each formula 5 ml is melted in 500 ml of pure aqua, and then  
the sugar cane seeds are soaked in the formula for 24 hours. This  
study is in addition to 3 formulas compared to control (not given a  
consortium of microorganisms) so there are 3 sample treatments  
namely: S0 (control), S1 (with formula 1). S2 (with formula 4) and  
8
5
6
.
Chohan M, Pahnwar R, Qazi B, Junejo S, Unar G, Arain M, Talpur U  
Quantitative and qualitative parameters of sugarcane variety hoth-300  
.
as affected by different levels of npk applications. Journal of Animal &  
Plant Sciences, 2012; 22: 1060-1064.  
S3 (with formula 5). Then observed the development of growing7. Elena P., Galina O. Information resources of soil nanoparticles  
shoots. The results obtained are then planted on the same planting  
medium as the composition of sand, soil and manure 1: 1: 1 in gasil  
which can be different between the treatments of Sample 0 (S0).  
Based on this Test and Formulation, a formula is obtained that  
can increase the speed of growth of sugarcane seedlings and is  
expected to be used for biological fertilizer for sugarcane, ant this  
is in agreement with Pereira et al. [8]. However, it still needs to be  
studied in more detail whether the formula is true in the soil  
biology laboratory, then proceed with the test of the best formula  
content which is named RIM (Refresh Microorganism) and get the  
results of the Plant Disease Laboratory test.  
chemistry. International Journal of Applied and Physical Sciences,  
2018; 4(2): 50-58. doi: https://dx.doi.org/10.20469/ijaps.4.50003-2.  
Pereira W, Sousa JS, Schultz N. Sugarcane productivity as a function  
of nitrogen fertilization and inoculation with diazotrophic plant  
growth-promoting bacteria. Sugar Tech. 2019; 21: 7182.  
8
.
4
Conclusion  
The formulation obtained was most suitable for the growth of  
sugarcane  
Sacharomyces (S), Azospirillum sp (A), Azotobacter (Z), Rhizobium  
R), Pseudomonas (P), Bacillus (B) and Trichoderma sp, which is  
seedlings,  
formulations  
of  
microorganisms  
(
formulated in a microorganism technology with the popular name  
Refresh Microorganism (RIM). Still need to be continued research  
trials using this RIM microorganism technology for the growth and  
production of sugar cane on agricultural land.  
Acknowledgment  
Submission of thanks to DRPM Ristekdikti for providing  
National Scale Applied Research funding for Fiscal Year 2019 and  
2
020.  
Ethical issue  
Authors are aware of, and comply with, best practice in  
publication ethics specifically with regard to authorship (avoidance  
of guest authorship), dual submission, manipulation of figures,  
competing interests and compliance with policies on research  
ethics. Authors adhere to publication requirements that submitted  
work is original and has not been published elsewhere in any  
language.  
Competing interests  
The authors declare that there is no conflict of interest that  
would prejudice the impartiality of this scientific work.  
Authors’ contribution  
All authors of this study have a complete contribution for data  
collection, data analyses and manuscript writing.  
References  
1
2
3
.
.
.
Awoke Mengesha T. A review on challenges and opportunities of  
water hyacinth. Journal of Life Science and Biomedicine, 2017; 7(5):  
5
1-61.  
Omrani A, Shiekhdavoodi M. and Shomeili M. Spatial distribution of  
soil penetration resistance in sugarcane ratoon fields. Journal of Life  
Science and Biomedicine, 2013; 3(5):328-334.  
Sudiarso and Ririen Prihandarini. The effect of biofertilizer and  
inorganic fertilizer on the vegetative growth of sugarcane (saccharum  
of icinarum). Journal of Applied and Physical Sciences, 2018; 4(1): 8-  
1
3.  
4
.
Chernenok AKV, Kurishbayev A. and Nurmanov Y. Diagnostics and  
optimization of crops’ nitrogen nutrition in rainfed conditions of the  
northern kazakhstan,'' Journal of Applied and Physical Sciences, 2015;  
1
: 1-8. doi: 10.20474/-japs1.1.1.  
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